Subunit Vaccine Preparation of Bovine Rotavirus and Its Efficacy in Mice
نویسندگان
چکیده مقاله:
Background: Rotaviruses (RV) are important viral diarrheal agents in calves. Vaccination is an optimum measure to prevent bovine rotaviruses (BRV) infection. However, little research on BRV VP7 vaccine has been done and currently there is no BRV vaccine. Objective: To prepare a subunit vaccine of BRV and investigate its efficacy. Methods: Total RNA was extracted from MA104 cells infected with bovine rotavirus (BRV) strain GSB01. BRV VP7 gene was amplified using real time fluorescence quantitative PCR (qPCR). The pEASY-T3-VP7 plasmid was digested using Hind Ⅲ and BamHI restriction endonucleases, then recombined into the prokaryotic expression vector pET32a. The pET32a-VP7 and pET32a-VP7-LTB (heatlabile enterotoxin B subunit) were transformed into BL21 (DE3) competent cells of Escherichia coli, respectively, and induced with IPTG, then analyzed using SDS-PAGE. Sixty mice were randomly divided into three groups (n=20). Group A mice was used as His-tag control and mice in group B and C were inoculated with pET32a-VP7 and pET32a-VP7-LTB, respectively. VP7 IgG antibody titers and protection efficiency of pET32a-VP7-LTB were further determined in neonatal mice challenged with GSB01 BRV strain. Results: SDS-PAGE analysis showed that the pET32a-VP7 was highly expressed in the BL21 (DE3) cells. PET32a-VP7 and pET32a-VP7-LTB protein could promote VP7 IgG antibody titer（8.33×103 vs. 17.26×103）in mice. Immunization protection ratios of pET32a-VP7 and pET32a-VP7-LTB proteins in the neonatal mice were 86.4% and 91.7%, respectively. Conclusion: The fusion protein of pET32a-VP7- LTB had excellent immunogenicity and protected mice from BRV infection. Our findings can be used for further developing of a high-efficiency subunit vaccine of BRV.
منابع مشابه
subunit vaccine preparation of bovine rotavirus and its efficacy in mice
background: rotaviruses (rv) are important viral diarrheal agents in calves. vaccination is an optimum measure to prevent bovine rotaviruses (brv) infection. however, little research on brv vp7 vaccine has been done and currently there is no brv vaccine. objective: to prepare a subunit vaccine of brv and investigate its efficacy. methods: total rna was extracted from ma104 cells infected with b...
متن کاملSubunit Vaccine Preparation of Bovine Rotavirus and Its Efficacy in Mice.
BACKGROUND Rotaviruses (RV) are important viral diarrheal agents in calves. Vaccination is an optimum measure to prevent bovine rotaviruses (BRV) infection. However, little research on BRV VP7 vaccine has been done and currently there is no BRV vaccine. OBJECTIVE To prepare a subunit vaccine of BRV and investigate its efficacy. METHODS Total RNA was extracted from MA104 cells infected with ...
متن کاملUse of Sodium Alginate Adjuvant in Preparation of a Formalin Killed Vaccine Against Infectious Bovine Rhinotracheitis
متن کامل
Rotavirus vaccine efficacy in African and Asian countries.
The report by George Armah and colleagues (Aug 21, p 606) on the effi cacy of a pentavalent rotavirus vaccine in sub-Saharan Africa is encouraging news for the control of rotavirus gastroenteritis in African children. Interestingly, the same vaccine is about 50% less effi cacious than reported in some developed countries. Although the absolute proportion of severe diarrhoeal disease prevented i...
متن کاملthe survey of the virtual higher education in iran and the ways of its development and improvement
این پژوهش با هدف "بررسی وضعیت موجود آموزش عالی مجازی در ایران و راههای توسعه و ارتقای آن " و با روش توصیفی-تحلیلی و پیمایشی صورت پذیرفته است. بررسی اسنادو مدارک موجود در زمینه آموزش مجازی نشان داد تعداد دانشجویان و مقاطع تحصیلی و رشته محل های دوره های الکترونیکی چندان مطلوب نبوده و از نظر کیفی نیز وضعیت شاخص خدمات آموزشی اساتید و وضعیت شبکه اینترنت در محیط آموزش مجازی نامطلوب است.
Virus-like particles as a rotavirus subunit vaccine.
Rotavirus subunit vaccines are being evaluated for use in humans. The virus-like particles (VLPs) for these vaccines are produced in insect cells coinfected with combinations of baculovirus recombinants expressing bovine RIF VP2 and simian SA11, VP4, VP6, or VP7 rotavirus proteins. VLPs were administered parenterally to mice and rabbits, and the immunogenicity and protective efficacy of the vac...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 12 شماره 3
صفحات 188- 197
تاریخ انتشار 2015-09-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023